SKU: 11701128707

Human SEMA5A (Semaphorin 5A) ELISA Kit

Sale price$267.75 Regular price$297.50
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Description

Human SEMA5A (Semaphorin 5A) ELISA KitThe Human (SEMA5A) Semaphorin 5A ELISA Kit measures Semaphorin 5A in samples. The plate has been pre coated with Human SEMA5A antibody. SEMA5A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA5A Antibody is added and binds to SEMA5A in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SEMA5A antibody. After incubation unbound Streptavidin HRP is washed away during a

The Human (SEMA5A) Semaphorin 5A ELISA Kit measures Semaphorin 5A in samples. The plate has been pre-coated with Human SEMA5A antibody. SEMA5A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA5A Antibody is added and binds to SEMA5A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA5A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SEMA5A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

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SKU: 11701128707

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