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Ships within 48 hours · Estimated delivery Jul 12 - Jul 17
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Description
Mouse TGF-Beta2 ELISA KitThe Mouse TGF Beta2 ELISA kit is a This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre coated with TGFb2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to TGFb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
The Mouse TGF-Beta2 ELISA kit is a This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TGFb2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TGFb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TGFb2 in the samples is then determined by comparing the OD of the samples to the standard curve.
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