Human CSF2RBETA (Colony Stimulating Factor 2 Receptor Beta) ELISA Kit

Human CSF2RBETA (Colony Stimulating Factor 2 Receptor Beta) ELISA KitThe Human CSF2RBETA (Colony Stimulating Factor 2 Receptor Beta) ELISA Kit measures CSF2RBeta in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CSF2RB antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound

The Human CSF2RBETA (Colony Stimulating Factor 2 Receptor Beta) ELISA Kit measures CSF2RBeta in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CSF2RB antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with CSF2RB bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CSF2RB in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.