Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 8 - Jul 13
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Rat NQO1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with NADH Dehydrogenase, Quinone 1 (NQO1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of NADH Dehydrogenase, Quinone 1 (NQO1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat NADH Dehydrogenase, Quinone 1 | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Quinone NADH dehydrogenase 1, also known as NQO1, is the enzyme encoded by the NQO1 gene. This protein-encoding gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a two-electron reductase. This FAD-binding protein forms a homodimer and performs the two-electron reduction of quinone to hydroquinone and other redox dyes. It prefers short-chain acceptor quinones such as ubiquinone, benzoquinone, juglone, and duroquinone. This gene has an important paralog, NQO2. This protein is located in the cytosol. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.4 ★★★★★
Based on 1446 reviews
Sort
Product Reviews
★★★★★ 5
BLEW ME AWAY! Loved every moment
Format: Kindle, Format: Kindle
My new FAVORITE BOOK! Priestess by Kara Reynolds blew me away from start to finish. This is a beautiful story about feminine power, sacrifice, self-worth, friendship, found family, healing and love. And the SLOW burn romance is excruciating and exquisite in the best way (“I am beyond temptation, I have been tempted and I have surrendered”). It was especially wonderful to find a fantasy novel with an FMC in her late 30s that is very relatable. It explores the topics of finding love later in life, fertility and the struggles women have with attaching self-worth with motherhood (My mother says all women are mothers in different ways, that we all give birth, just not all to children. Some women give birth to revolutions, to movements, to sanctuary, to art, to brilliance.”) GAHHH love this. I felt so seen. The twists and turns had me on the edge of my seat, dying to turn the pages faster to know what happens and at the same time wanting to slow down and enjoy Reynolds beautiful prose and savor each chapter. The ending was perfect and the authors note made me cry. I CANNOT wait to read Illuminator and Pilgrimess which will be out April 28th!!!! Kara Reynolds is def on my auto-buy list 💚
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 3, 2026
★★★★★ 5
Take a jaunt to Tintar, a city of blue abalones and black stone set above the angry sea...
Format: Paperback
Edie is a clever, gutsy heroine, who is lucky to be surrounded by a crew of likewise interesting ladies. Their tender moments in the bath and at the bar alike were very lovely, and the themes of female friendship were refreshing.
My favorite parts had to do with the magic of this world, which is very novel. Often there's elemental magic, earth gets the short end of the stick. Not so here. The power of earth in this seaside landscape cannot be underestimated, and I really enjoyed that twist. Earth signs, at least, will rejoice in seeing their element treated with real consideration.
All of the scenes of magic and old gods were A+, and I loved the weirdness of the rocks first coming to her in the forest, then erupting from the ground to let her know without a doubt what her strength was, and finally forming majestic creatures to rescue her new home—all based on the women who had supported and loved her.
The final conflict was excellent. There was a real sense that Edie was going to sacrifice something that she did not want to let go of, which you don't get that often. (Plus, it was pretty funny when a certain someone got tossed over a wall.)
Finally, given the themes of womanhood in this book, I am going to pull a word from the big reviewers' lexicon and say that this book is /timely/. What it says about what women are and are not, how it explores Edie's feelings about her body, were thoughtful and illuminating and, yes, timely and given the political climate we are now in—I will even add /necessary/ to that list.
It's a rich, femme-centric fantasy epic that made me think about womanhood (and motherhood) in a new light.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 26, 2024
★★★★★ 5
Woman's experience and the power of female friendships
Format: Kindle
This story was amazing, and had several elements currently lacking from the genre. It was mature, wise, experienced, and empathetic. It was wonderfully representative of women's experience, with the bonds and friendships between them serving as the balm for life's hurts.
**Spoiler alert/warning: Reynolds tackled SA, infertility, teenage pregnancy, and more. Her representation of infertility is the most true-to-form capture of that awful journey that I've ever seen in literature (AND it wasn't "cured" by the love of the "right" man - something that drives me crazy in 99.99999% of stories that try to touch on infertility). She even included the realness of the older infertile woman's response to the teenage pregnancy in a raw and unfiltered way. Another review said it but I'll back it up: Kara knows. And she shows you that she knows.
Thank you for your empathetic writing, Kara Reynolds. Thank you for seeing women and our experiences and representing us on the page. I was touched, moved, inspired, and comforted by the power of female friendships and found family. The romance was gorgeous, even in its predictability. Because it was clear that the true loves of her life were those enduring women who walked life alongside her. Amazing story.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 24, 2025
★★★★★ 4
Girl a struggle that I couldn't not finish.
Format: Kindle
Keep writing Kara girl. Maybe shorten something. But I enjoyed the story. I did not enjoy the uncertainty because it was never uncertain. I enjoy the story though.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 13, 2025
★★★★★ 5
OUTSTANDING
Format: Kindle
This was very, very good. The world is vast and characters are complex. There is a good plot with a whole lot going on. This is well written. Good twists and turns and some heart breaking moments. You will love these characters, they have heart and loyalty. I am hoping that there will be several more books. We've yet to see anything from the Sea Court but only a mention of them here and there. The Wood Court was given a quick couple of scenes, and only as far as some warriors, we've yet to enter their court and the Shadow Court, I'm not sure if they will be a force for good or bad, but they definitely will play a much bigger role moving forward. This is primarily the Ice and Air Courts. Told in multiple views, which I loved, it gives you a chance to see things from different eyes. There's alot of political maneuvering and deception. I loved it and will pick up the next book as it becomes available. If you like The Fae and the courts, you should love this. I think the author has mucn in store for us.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 8, 2020