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Description
Mouse TL ELISA KitProduct Specification Sensitivity 9. 375ng ml Species Reactivity Mouse Theory This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin labeled anti TC antibody. Free components are washed away. HRP streptavidin (SABC) is
Product Specification
| Sensitivity | 9.375ng/ml | ||||||||||||||||||||||||||||||||||
| Species Reactivity | Mouse | ||||||||||||||||||||||||||||||||||
| Theory | This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre-coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin-labeled anti-TC antibody. Free components are washed away. HRP-streptavidin (SABC) is added, and biotin specifically binds to streptavidin to form an immune complex. The unbound conjugate was washed off and TMB chromogenic substrate was added. The TMB became blue under the catalysis of horseradish peroxidase (HRP) and turned yellow after the reaction stop solution was added. The OD value was measured at 450nm with a microplate reader. Using curve equation software, the OD450 value of the sample was compared to the standard curve to determine the concentration of TC in the sample. There is an inverse proportion between the concentration of the target substance and the OD450 value. | ||||||||||||||||||||||||||||||||||
| Synonym | Mouse TC (Total cholesterol) ELISA Kit | ||||||||||||||||||||||||||||||||||
| Detection Type | Competition Law | ||||||||||||||||||||||||||||||||||
| Composition |
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| General Notes | 1. When using different kits, it is necessary to mark them first to prevent the components from being mixed, resulting in experimental failure. 2. After the kit is opened, please refer to the component storage conditions table for the storage conditions of the enzyme plate and standard product (the activity will decrease after moisture). 3. Please use a sterile disposable tip to suck the reagent. After use, the reagent bottle cap must be tightened to prevent microbial contamination and evaporation. 4. When washing the plate manually, add the tip or dropper of the washing liquid and do not touch the hole of the enzyme labeled plate. Inadequate washing or contamination tend to cause false positives and high backgrounds. 5. During the testing process, please prepare the reagents required for the next experiment in advance, and add the reagents to the plate holes in time after washing the plate to prevent the plate holes from drying out and causing testing failure. 6. Do not use reagents from other batches of kits or reagents from other sources for this kit without confirmation. 7. Do not reuse disposable tips to avoid cross-contamination. 8. After the sample addition is completed, the film is applied to prevent the sample from evaporating during the incubation process, and the incubation process is completed at the recommended temperature. 9. Please wear laboratory coats, masks, gloves, etc. during the test, and do a good job of protection. Especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations. |
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| Instructions | Serum, plasma, cell culture supernatant, cell lysate and other fluid samples | ||||||||||||||||||||||||||||||||||
| Storage Temp. | Unopened kit, stored at 2-8 °C, shelf life for one year. | ||||||||||||||||||||||||||||||||||
| Test Range | 15.625-1000ng/ml |
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4.2 ★★★★★
Based on 2496 reviews
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Product Reviews
★★★★★ 5
My dog loves this toy!
Color: Green
I was NOT expecting my dog to like this toy, but she loves it! The legs are stretchy and the body vibrates. The battery lasts about a month.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 8, 2026
★★★★★ 5
Great interactive toy. Worth the price.
Color: Blue
Our Lab absolutely loves it. She throws it all over the house. It’s so fun to watch her play with it. It’s by far her favorite toy right now. It’s easy to charge and came with charging cable. It has a button to turn off and on and a couple different speeds. My parents got her a different one for Christmas and since she loved it so much we decided to get her another one. It’s pretty durable too. She normally rips through toys pretty quickly and both of the ones she has are still in good shape. No rips or tears. She hasn’t tried just chewing on it like she does her squeaky toys. She just throws it all around the house and chases it.
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Reviewed in the United States on March 17, 2026
★★★★★ 4
Fun, enticing dog toy. As stated in description it won’t hold up for aggressive chewers
Color: Blue
Rating this based on description. My dog loved this toy. It was smaller than I had pictured in my head, but that was on me. I did read the description ahead of time, which clearly states this is not for aggressive chewers. My dog is trained not to rip toys apart so I wanted to give this a shot, but i guess just her jaw strength and teeth were too much. She carried it everywhere. I turned it on periodically. The movements are small, but enough for her to be captivated with the toy. It did mostly survive for about a day before I needed to take it away due to the Velcro opening. Once it started to pull apart she did chew a piece of the Velcro off. It might be helpful with a flap over design covering the Velcro. I have a singing Santa decoration like this where the Velcro is covered up and I have to pull it back a little to get to the Velcro opening. The gaps on the sides of the closure are a bit to large making it tempt my dog to pull and open it up.
I wish there was a more durable option that could hold up to aggressive chewers, but this product was clear about that in the description. I think the opening to the batteries could use some improvement. Granted I do not have the perfect answer to fix this, but I am also not a toy designer.
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Reviewed in the United States on April 19, 2026
★★★★★ 5
A Toy Ball for All Seasons!
Style: Small Ball, Size: 1 Count, Style: Small Ball, Size: 1 Count
Oh my gosh! I’m so happy to find the almost exact ball my 5 1/2 pound 9 month old Morkie, Charlie, that mommy lost at the campgrounds! It was his favorite toy and although he has others, he loved this one above all others, so to find another one was an answer to my prayers. The difference is so minor and in a good way. It is very durable as Charlie has very sharp teeth, but this one is just a bit softer making it easier to hang on to playing fetch. But for Charlie, the size is the same, the squeekiness is the same, the color is the same (yes they can see some colors) and he went into a jumping frenzy when I took it out of the package and the price was unbelievably inexpensive for such a high quality toy! Thank you! Thank you! Thank you! Good mommy! ( I’m ordering another one as a back up, jic!)
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Reviewed in the United States on May 15, 2026
★★★★★ 5
Great investment.
Style: Small Ball, Size: 2 Count
Dogs love them. Durable. Chew worthy for a beagle and a German shepherd. You cannot smell the flavor imparted but the pups definitely can. Always purchase the amount you need so each dog can have one.
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Reviewed in the United States on May 19, 2026
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