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Description
Rat PGC-1α ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotinylated detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against peroxisome proliferator activated receptor gamma coactivator 1-alpha (PGC-1α). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of peroxisome proliferator activated receptor gamma coactivator 1-alpha (PGC-1α) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Peroxisome proliferator activated receptor gamma coactivator 1-alpha ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Peroxisome proliferator-activated receptor gamma cofactor 1-alpha (PGC-1α) is a protein encoded by the PPARGC1A gene. PGC-1α is a master regulator of mitochondrial biogenesis and hepatic glucose production, inducing increased expression of glucose-dependent genes. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.8 ★★★★★
Based on 1955 reviews
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Product Reviews
★★★★★ 5
Best steamer on market hands down
Size: Standard, Size: Standard
If you want to really deep clean your home then this will be your best friend. Comes with several different attachments which is great. It’s light weight so not heavy to carry around. So easy to put together or change attachments. They give you a measuring cup for the exact amount of water you need with a funnel so no mess putting water in. Heats up super fast which is awesome. Like i said light weight but strong steamer. The first thing i used it on was my oven which is 8 or 9 years old. From cooking especially holidays there was grease etc on it. I used the oven cleaners that say removes grease with one wipe Nope it those products actually ruined top of my stove made it dull and even other parts almost a rust look which I am not happy with. Wish I knew about this amazing machine before I ever used the store bought oven cleaner. Like I said it comes with several attachments ones for steaming your floors amazing. An attachment for grout also did an amazing job I was shocked my shower looks brand new my floors look brand new also. Another attachment is for steaming shower doors, walls, furniture and clothes works awesome. Another attachment for hard to reach places super amazing. Oh and the handle can come apart so you can make it long for when your steaming floors then make it smaller to carry around to do all the other steaming you want to do. Best part is that it didn’t break the bank the price was well worth it. I first saw this steamer on TickToc like with other items I have bought. But my go to place to see if these items are on Amazon cause Amazon is my go to place to shop, also the prices are always cheaper and you can get it in 2 days or other things I bought within hours. Where tik tok it takes weeks to get and always more expensive. So thank you Amazon for being an amazing company. If i could give more stars for this steamer I would give it a 20 star that’s how great it works. One thing I forgot to do was take a picture of my stove before but I did after. You can see where those store products ruined areas I mentioned. That was not caused by this amazing product.
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Reviewed in the United States on October 16, 2025
★★★★★ 5
Great all-in-one steam cleaner
Size: Standard
The Schenley 19-in-1 Steam Mop is super versatile and easy to use. It heats up quickly and the steam is strong enough to clean floors, grout, and bathroom surfaces without any chemicals.
I love how easily it switches to a handheld steamer—feels like multiple tools in one. The build is sturdy and it maneuvers well.
Overall it’s a great value and works really well.
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Reviewed in the United States on April 27, 2026
★★★★★ 5
Great Lightweight Steam Mop!
Size: Compact, Size: Compact
I’m really happy with the Schenley Steam Mop. It heats up in about 15 seconds and is ready to go almost instantly. It works well on my hardwood and tile floors, removing everyday dirt without needing any chemicals.
It’s lightweight, easy to maneuver, and I love that it stands on its own. The washable pads and adjustable steam modes are a nice bonus. The water tank is a bit small, but for regular cleaning it’s perfect.
Great value and very convenient for quick, effective cleaning!
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Reviewed in the United States on February 14, 2026
★★★★★ 4
Great versatile tool, not self-standing and missing a few accessories
Size: Standard
This is a great lightweight and versatile steam mop with good attachments. I would have given it five stars, but it is NOT self standing, does not auto-shut off, and does not have a built in cord wrap. You must manually buy the cord and then use their provided Velcro wrap to secure it in place. It also did not come with a storage bag for attachments, a cup to fill water tank, or a carpet glider.
It’s water tank lasts a long time during steaming and when combined with a floor cleaner, I apply directly to the floor, works wonders for our two large dog’s footprints on our 1000 sq. ft. of laminate flooring. One to two tanks of water clean the entire square footage.
After researching, I cannot find a better steam mop, with attachments that has all the features I want at $100. It also looks very sleek next to our Dyson. I would recommend.
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Reviewed in the United States on June 24, 2025
★★★★★ 5
The best!
Size: Standard, Size: Standard
This is definitely the best steam mop I have ever tried. It is easy to use and easy to assemble. I like the cord length. It covered my entire living room, kitchen and hallway. I just had to switch once! It comes with different attachments which you can use in your kitchen, and to clean windows etc. The mop pads are easy to clean and machine washable. It is definitely a must have if you want a clean, germ free home. Definitely recommended.
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Reviewed in the United States on May 23, 2026
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